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Ceritalah Doktor [Tell Me Doctor]

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Showing posts with label Agar. Show all posts
Showing posts with label Agar. Show all posts

Thursday, December 4, 2008

MacConkey agar / McConkey agar concept

What is this for?
designed to grow Gram-negative bacteria and stain them for lactose fermentation.
distinguishes those Gram-negative bacteria that can ferment the sugar lactose from those that cannot

Contents
It contains :
  • bile salts and crystal violet dye to inhibit most Gram-positive bacteria
  • neutral red dye to stains microbes fermenting lactose
  • lactose and peptone.

The concept :

  • bacteria that can ferment sugar lactose will produce acid
  • lowers the pH
  • appearance of red/pink colonies.

  • bacteria that cannot ferment sugar lactose will use peptone
  • producing ammonia
  • raise pH
  • appearance of white / colourless colonies

Macconkey agar with lactose (left) and non-lactose (right) fermenters
at 5:44 AM 0 comments Labels: Agar
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